Objective: To determine the diagnostic accuracy of Magnetic Resonance spectroscopy in differentiating neoplastic from non-neoplastic enhancing brain lesions taking histopathology as gold standard. Design: Cross sectional study Patients and Methods: A total of 51 patients with enhancing lesions detected on MRI brain contrast studies were included in this study. MRS was performed. On MRS, lesion was categorized and final diagnosis was made on histopathology results. All the information was recorded into predesigned proforma. Results: The average age of the patients was 40.37±11.56 years. Sensitivity, specificity, PPV, NPV and accuracy of MRS was 87.5%, 93.3%, 95.5%, 89.7% and 92.1% respectively. Conclusion: Magnetic resonance spectroscopy can readily help in differentiating neoplasm from non-neoplastic brain tumors, thus an invasive brain biopsy procedure can be avoided.